Head of ACF: Ferhan Ayaydin PhD

FCBI ACF provides high-throughput and high-resolution imaging services, detection of cell surface markers and intracellular markers, as well as the possibility for sorting cells based on their expressed protein markers for cellular and immunology studies. Accordingly, the mainstay of the FCBI ACF includes top quality correlative light and electron microscopy (CLEM) representing a highly advanced technological platform for the molecular analysis of biological specimens and a high-performance flow cytometer and cell sorter capable of complex cell sorting based on cell surface molecular markers and high-sensitivity analysis. Thus, one unit of the core facility enables the users to perform 3D array tomography electron microscopy with a wide range of analytic applications and possibilities for in situ experiments, while the other unit offers the capabilities of a flow cytometer with advanced cell sorter capacities. The main goal of this ACF is to provide for high-throughput and high-resolution imaging, cell surface and intracellular marker detection as well as the possibility for sorting cells based on their expressed protein markers for cellular and immunology studies.

Instruments of ACF

The major available techniques at FCBI ACF include conventional (wide-field, confocal) and advanced (super resolution) light microscopy, scanning electron microscopy optimized for biological samples (including array tomography and correlative light and electron microscopy), an advanced cell sorter facility and a molecular biology facility.

Light Microscopy

             Leica Stellaris laser scanning super-resolution confocal microscope

            Zeiss LSM880 laser scanning confocal microscope

            Olympus FV10i self-contained confocal microscope

            Oxford Nanoimager super resolution microscope

Electron Microscopy

            Zeiss Sigma 300 scanning electron microscope

            RMC Powertome with ATUMtome tape collection

            Quorum Q150R Plus rotary pumped carbon coater

Cell Sorter

            BD FACS Aria Fusion

Molecular Biology Support