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HCEMM-BRC Single Cell Omics Advanced Core Facility

Head of Advanced Core Facility

Zsuzsanna Darula PhD

Head of ACF


Head of Single Cell Omics Advanced Core Facility, BRC-HCEMM, 2020-

Head of Laboratory of Proteomics Research, Biological Research Centre, Eötvös Loránd Research Network 2020-

Visiting scientist, Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus 2018-2019

Staff Scientist, Biological Research Centre, Eötvös Loránd Research Network 1999-2020

Visiting scientist, Department of Pharmaceutical Chemistry, School of Pharmacy, UCSF 2001


Ph.D. (2000) Faculty of Pharmacy, University of Szeged, Szeged, Hungary

M.Sc. (1996) in Chemistry, Faculty  of Science, University of Szeged, Szeged, Hungary


number of publications: 62

number of independent citations (MTMT):  1362

Hirsch-index (MTMT): 20


2013 – III. prize, Qualitas Biologica Publication Award (Hungary)

2011 – János Bolyai Researh Scholarship

2010 – I. prize, Qualitas Biologica Publication Award (Hungary)

1999 – Soros Predoctoral Scholarship

1999 – Predoctoral Scholarship of the Foundation for the Hungarian Peptide and Protein Research


2019 – European Proteomics Association

2011 – Hungarian Society for Separation Sciences

2000 – Hungarian Biochemical Society

2000 – Federation of European Biochemical Societies


  1. J. Chalkley, K. F. Medzihradszky, Z. Darula, A. Pap, and P. R. Baker, “The effectiveness of filtering glycopeptide peak list files for Y ions,” MOLECULAR OMICS, vol. 16, no. 2, pp. 147–155, 2020.
  2. Kurilla, T. Toth, L. Dorgai, Z. Darula, T. Lakatos, D. Silhavy, Z. Kerenyi, and G. Dallmann, “Nectar- and stigma exudate-specific expression of an acidic chitinase could partially protect certain apple cultivars against fire blight disease,” PLANTA, vol. 251, no. 1, 2020.
  3. Pap, E. Tasnadi, K. F. Medzihradszky, and Z. Darula, “Novel O-linked sialoglycan structures in human urinary glycoproteins,” MOLECULAR OMICS, vol. 16, no. 2, pp. 156–164, 2020.
  4. Szabo, K. Karaszi, R. Romero, E. Toth, A. Szilagyi, Z. Gelencser, Y. Xu, A. Balogh, G. Szalai, P. Hupuczi, B. Hargitai, T. Krenacs, E. Hunyadi-Gulyas, Z. Darula, K. A. Kekesi, A. L. Tarca, O. Erez, G. Juhasz, I. Kovalszky, Z. Papp, and N. G. Than, “Proteomic identification of placental protein 1 (PP1), PP8, and PP22 and characterization of their placental expression in healthy pregnancies and in preeclampsia,” PLACENTA, vol. 99, pp. 197–207, 2020.
  5. Baba, I. Valkai, N. M. Labhane, L. Koczka, N. Andrási, É. Klement, Z. Darula, K. F. Medzihradszky, L. Szabados, A. Fehér, G. Rigó, and Á. Cséplő, “CRK5 Protein Kinase Contributes to the Progression of Embryogenesis of Arabidopsis thaliana.,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 24, 2019.
  6. Baba, N. Andrási, I. Valkai, T. Gorcsa, L. Koczka, Z. Darula, K. F. Medzihradszky, L. Szabados, A. Fehér, G. Rigó, and Á. Cséplő, “AtCRK5 Protein Kinase Exhibits a Regulatory Role in Hypocotyl Hook Development during Skotomorphogenesis.,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 14, 2019.
  7. Darula, A. Pap, and K. F. Medzihradszky, “Extended Sialylated O-Glycan Repertoire of Human Urinary Glycoproteins Discovered and Characterized Using Electron-Transfer/Higher-Energy Collision Dissociation,” JOURNAL OF PROTEOME RESEARCH, vol. 18, no. 1, pp. 280–291, 2019.
  8. Hevér, Z. Darula, and K. F. Medzihradszky, “Characterization of Site-Specific N-Glycosylation.,” METHODS IN MOLECULAR BIOLOGY, vol. 1934, pp. 93–125, 2019.
  9. Laurinyecz, V. Vedelek, A. L. Kovács, K. Szilasi, Z. Lipinszki, C. Slezák, Z. Darula, G. Juhász, and R. Sinka, “Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm.,” PLOS GENETICS, vol. 15, no. 2, 2019.
  10. R. Schmitt, R. Henderson, A. Barrett, Z. Darula, A. Issaian, A. D’Alessandro, N. Clendenen, and K. C. Hansen, “Mass spectrometry-based molecular mapping of native FXIIIa cross-links in insoluble fibrin clots,” JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 294, no. 22, pp. 8773–8778, 2019.
  11. É. Csősz, B. Márkus, Z. Darula, K. Medzihradszky, J. Nemes, J. Tőzsér, C. Kiss, and I. Márton, “Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population.,” FEBS OPEN BIO, vol. 8, pp. 556–569, 2018.
  12. Á. Dancs, K. Selmeczi, I. Bányai, Z. Darula, and T. Gajda, “Increasing the histidine ‘density’ in tripodal peptides by gradual N-functionalization of tris(2-aminoethyl)amine (tren) with l-histidyl units: The effect on zinc(II) complexes,” INORGANICA CHIMICA ACTA, vol. 472, no. March, pp. 174–183, 2018.
  13. Darula and K. Medzihradszky, “Analysis of Mammalian O-Glycopeptides-We Have Made a Good Start, but There is a Long Way to Go.,” MOLECULAR & CELLULAR PROTEOMICS, vol. 17, no. 1, pp. 2–17, 2018.
  14. Dory, E. Hatzimasoura, B. M. Kállai, S. K. Nagy, K. Jäger, Z. Darula, T. V. Nádai, T. Mészáros, E. Lopez Juez, B. Barnabás, K. Palme, L. Bögre, F. A. Ditengou, and R. Dóczi, “Coevolving MAPK and PID phosphosites indicate an ancient environmental control of PIN auxin transporters in land plants,” FEBS LETTERS, vol. 592, no. 1, pp. 89–102, 2018.
  15. Migh, T. Gotz, I. Foldi, S. Szikora, R. Gombos, Z. Darula, K. Medzihradszky, J. Maleth, P. Hegyi, S. Sigrist, and J. Mihaly, “Microtubule organization in presynaptic boutons relies on the formin DAAM,” DEVELOPMENT, vol. 145, no. 6, 2018.
  16. T. Nandor, R. Roberto, L. T. Adi, A. K. Katalin, X. Yi, X. Zhonghui, K. Juhasz, B. Gaurav, J. L. Ron, Z. Gelencser, J. Palhalmi, H. C. Tzu, A. G. Balazs, O. Laszlo, D. Amanda, A. Szecsi, H.-G. Eva, D. Zsuzsanna, S. Attila, E. Katalin, S. Szilvia, T. Vanessa, E.-A. Haidy, L. Christopher, B. Andrea, S. Gabor, L. Susan, T. Olga, D. Zhong, K. Ilona, F. Andras, M. Hamutal, D. Sorin, S. H. Sonia, C. Tinnakorn, K. Manuel, K. Martin, E. Offer, J. B. Graham, J. K. Chong, J. Gabor, and P. Zoltan, “Integrated Systems Biology Approach Identifies Novel Maternal and Placental Pathways of Preeclampsia,” FRONTIERS IN IMMUNOLOGY, vol. 9, 2018.
  17. Pap, E. Klement, E. Hunyadi-Gulyas, Z. Darula, and K. Medzihradszky, “Status Report on the High-Throughput Characterization of Complex Intact O-Glycopeptide Mixtures,” JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 29, no. 6, pp. 1210–1220, 2018.
  18. Pap, A. Prakash, K. F. Medzihradszky, and Z. Darula, “Assessing the reproducibility of an O-glycopeptide enrichment method with a novel software, Pinnacle,” ELECTROPHORESIS, vol. 39, no. 24, pp. 3142–3147, 2018.
  19. Á. Dancs, N. V May, K. Selmeczi, Z. Darula, A. Szorcsik, F. Matyuska, T. Páli, and T. Gajda, “Tuning the coordination properties of multi-histidine peptides by using a tripodal scaffold: solution chemical study and catechol oxidase mimicking,” NEW JOURNAL OF CHEMISTRY, vol. 41, no. 2, pp. 808–823, 2017.
  20. Gabor, G. Cinege, G. Csordas, T. Torok, F. Medzihradszky, Z. Darula, I. Ando, and E. Kurucz, “Hemolectin expression reveals functional heterogeneity in honey bee (Apis mellifera) hemocytes,” DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, vol. 76, pp. 403–411, 2017.
  21. Gellen, K. Volgyi, B. Gyorffy, B. Balogh, Z. Darula, E. Hunyadi-Gulyás, P. Baracskay, A. Czurko, I. Hernadi, G. Juhasz, A. Dobolyi, and K. Kekesi, “Proteomic investigation of the prefrontal cortex in the rat clomipramine model of depression,” JOURNAL OF PROTEOMICS, vol. 153, pp. 53–64, 2017.
  22. Horvath, H. Kourova, S. Nagy, E. Nemeth, Z. Magyar, C. Papdi, Z. Ahmad, G. Sanchez-Perez, S. Perilli, I. Blilou, A. Pettkó-Szandtner, Z. Darula, T. Meszaros, P. Binarova, L. Bogre, and B. Scheres, “Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control,” EMBO JOURNAL, vol. 36, no. 9, pp. 1261–1278, 2017.
  23. Horváth, I. Tamás, A. Sipos, Z. Darula, B. Bécsi, D. Nagy, J. Iván, F. Erdődi, and B. Lontay, “Myosin phosphatase and RhoA-activated kinase modulate neurotransmitter release by regulating SNAP-25 of SNARE complex,” PLOS ONE, vol. 12, no. 5, pp. 1–23, 2017.
  24. Pap, K. Medzihradszky, and Z. Darula, “Using ‘spectral families’ to assess the reproducibility of glycopeptide enrichment: human serum O-glycosylation revisited,” ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 409, no. 2, pp. 539–550, 2017.
  25. Sipos, J. Iván, B. Bécsi, Z. Darula, I. Tamás, D. Horváth, K. Medzihradszky, F. Erdődi, and B. Lontay, “Myosin phosphatase and RhoA-activated kinase modulate arginine methylation by the regulation of protein arginine methyltransferase 5 in hepatocellular carcinoma cells,” SCIENTIFIC REPORTS, vol. 7, 2017.
  26. Darula, F. Sarnyai, and K. Medzihradszky, “O-glycosylation sites identified from mucin core-1 type glycopeptides from human serum,” GLYCOCONJUGATE JOURNAL, vol. 33, no. 3, pp. 435–445, 2016.
  27. Lorincz, Z. Lakatos, A. Varga, T. Maruzs, Z. Simon-Vecsei, Z. Darula, P. Benko, G. Csordas, M. Lippai, I. Ando, K. Hegedus, K. F. Medzihradszky, S. Takats, and G. Juhasz, “MiniCORVET is a Vps8-containing early endosomal tether in Drosophila,” ELIFE, vol. 5, 2016.
  28. Varga, O. Berkesi, Z. Darula, N. May, and A. Palagyi, “Structural characterization of allomelanin from black oat,” PHYTOCHEMISTRY, vol. 130, pp. 313–320, 2016.
  29. Bocsik, Z. Darula, G. Tóth, M. Deli, and M. Wollemann, “Transfer of opiorphin through a blood-brain barrier culture model,” ARCHIVES OF MEDICAL RESEARCH, vol. 46, no. 6, pp. 502–506, 2015.
  30. Darula and K. Medzihradszky, “Carbamidomethylation Side Reactions May Lead to Glycan Misassignments in Glycopeptide Analysis,” ANALYTICAL CHEMISTRY, vol. 87, no. 12, pp. 6297–6302, 2015.
  31. Á. Edit, Y. Ping, F. Ilona, D. Zsuzsanna, V. Erzsébet, L. Noémi, A. Ferhan, F. M. Katalin, D. Viktor, D. Dénes, and V. H. Gábor, “The B″ regulatory subunit of protein phosphatase 2A mediates the dephosphorylation of rice retinoblastoma‑related protein‑1,” PLANT MOLECULAR BIOLOGY, vol. 87, no. 1–2, pp. 125–141, 2015.
  32. Kobayashi, T. Suzuki, E. Iwata, N. Nakamichi, T. Suzuki, P. Chen, M. Ohtani, T. Ishida, H. Hosoya, S. Muller, T. Leviczky, A. Pettko-Szandtner, Z. Darula, A. Iwamoto, M. Nomoto, Y. Tada, T. Higashiyama, T. Demura, J. Doonan, M. Hauser, K. Sugimoto, M. Umeda, Z. Magyar, L. Bogre, and M. Ito, “Transcriptional repression by MYB3R proteins regulates plant organ growth,” EMBO JOURNAL, vol. 34, no. 15, pp. 1992–2007, 2015.
  33. Nagy, Z. Darula, B. Kallai, L. Bogre, G. Banhegyi, K. Medzihradszky, G. Horvath, and T. Meszaros, “Activation of AtMPK9 through autophosphorylation that makes it independent of the canonical MAPK cascades,” BIOCHEMICAL JOURNAL, vol. 467, no. 1, pp. 167–175, 2015.
  34. Darula and K. Medzihradszky, “Glycan Side Reaction May Compromise ETD-Based Glycopeptide Identification,” JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 25, no. 6, pp. 977–987, 2014.
  35. Perez-Salamo, C. Papdi, G. Rigo, L. Zsigmond, B. Vilela, V. Lumbreras, I. Nagy, B. Horvath, M. Domoki, Z. Darula, K. Medzihradszky, C. Koncz, L. Bogre, and L. Szabados, “The Heat Shock Factor A4A Confers SaltTolerance and Is Regulated by OxidativeStress and the Mitogen-Activated ProteinKinases MPK3 and MPK6,” PLANT PHYSIOLOGY, vol. 165, no. 1, pp. 319–334, 2014.
  36. Rigo, F. Ayaydin, O. Tietz, L. Zsigmond, H. Kovacs, A. Pay, K. Salchert, Z. Darula, K. Medzihradszky, L. Szabados, K. Palme, C. Koncz, and A. Cseplo, “Inactivation of Plasma Membrane-Localized CDPK-RELATED KINASE5 Decelerates PIN2 Exocytosis and Root Gravitropic Response in Arabidopsis.,” PLANT CELL, vol. 25, no. 5, pp. 1592–1608, 2013.
  37. Darula, J. Sherman, and K. Medzihradszky, “How to Dig Deeper? Improved Enrichment Methods for Mucin Core-1 Type Glycopeptides,” MOLECULAR & CELLULAR PROTEOMICS, vol. 11, no. 7, 2012.
  38. Kékesi, G. Juhász, A. Simor, P. Gulyássy, É. Szegő, É. Hunyadi-Gulyás, Z. Darula, K. Medzihradszky, M. Palkovits, B. Penke, and A. Czurkó, “Altered functional protein networks in the prefrontal cortex and amygdala of victims of suicide,” PLOS ONE, vol. 7, no. 12, 2012.
  39. Mot, M. Parvu, G. Damian, F. Irimie, Z. Darula, K. Medzihradszky, B. Brem, and R. Silaghi-Dumitrescu, “A ‘yellow’ laccase with ‘blue’ spectroscopic features, from Sclerotinia sclerotiorum,” PROCESS BIOCHEMISTRY, vol. 47, no. 6, pp. 968–975, 2012.
  40. Darula, R. Chalkley, A. Lynn, P. Baker, and K. Medzihradszky, “Improved identification of O-linked glycopeptides from ETD data with optimized scoring for different charge states and cleavage specificities,” AMINO ACIDS, vol. 41, no. 2, pp. 321–328, 2011.
  41. Molnar, G. Fabian, J. Klem, Z. Darula, E. Hunyadi-Gulyas, A. Medgyesi, K. Medzihradszky, and L. Puskas, “Removal of nonspecific binding proteins from cell and tissue extracts using 2-aminobenzimidazole-tethered affinity resin,” PHARMAZIE, vol. 66, no. 9, pp. 662–665, 2011.
  42. Sziksz, L. Himer, K. Kékesi, G. Juhász, A. Simor, P. Gulyássy, Z. Darula, B. Szebeni, G. Reusz, A. Szabó, T. Tulassay, and Á. Vannay, “Iszkémia-reperfúzió okozta akut vesekárosodás: új biomarkerek, terápiás célpontok azonosítása,” GYERMEKGYÓGYÁSZAT, vol. 62, no. 5, p. 235/3-235, 2011.
  43. Yu, E. Abraham, Z. Darula, D. Dudits, and V. Horvath, “The Role of Phosphorilation in the Function of RBR-interacting Phosphatase Subunit,” ACTA BIOLOGICA SZEGEDIENSIS, vol. 55, no. 1, pp. 189–193, 2011.
  44. Darula, R. Chalkley, P. Baker, A. Burlingame, and K. Medzihradszky, “Mass spectrometric analysis, automated identification and complete annotation of o-linked glycopeptides,” EUROPEAN JOURNAL OF MASS SPECTROMETRY, vol. 16, no. 3, pp. 421–428, 2010.
  45. Darula and K. Medzihradszky, “Affinity enrichment and characterization of mucin core-1 type glycopeptides from bovine serum,” MOLECULAR & CELLULAR PROTEOMICS, vol. 8, no. 11, pp. 2515–2526, 2009.
  46. Ronai, K. Kiraly, A. Szebeni, E. Szemenyei, Z. Prohaszka, Z. Darula, G. Toth, I. Till, B. Szalay, E. Kato, and I. Barna, “Immunoreactive endomorphin 2 is generated extracellularly in rat isolated l4,5 dorsal root ganglia by DPP-IV,” REGULATORY PEPTIDES, vol. 157, no. 1–3, pp. 1–2, 2009.
  47. Kupihar, Z. Timar, Z. Darula, D. Dellinger, and M. Caruthers, “An electrospray mass spectrometric method for accurate mass determination of highly acid-sensitive phosphoramidites,” RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 22, no. 4, pp. 533–540, 2008.
  48. Szemenyei, I. Barna, Z. Mergl, A. Keresztes, Z. Darula, E. Kato, G. Toth, and A. Ronai, “Detection of a novel immunoreactive endomorphin 2-like peptide in rat brain extracts,” REGULATORY PEPTIDES, vol. 148, no. 1–3, pp. 54–61, 2008.
  49. Zsigmond, G. Rigo, A. Szarka, G. Szekely, K. Otvos, Z. Darula, K. Medzihradszky, C. Koncz, Z. Koncz, and L. Szabados, “Arabidopsis PPR40 Connects Abiotic Stress Responses to Mitochondrial Electron Transport,” PLANT PHYSIOLOGY, vol. 146, no. 4, pp. 1721–1737, 2008.
  50. Kurucz, R. Markus, J. Zsamboki, M. K. Folkl, Z. Darula, P. Vilmos, A. Udvardy, I. Krausz, T. Lukacsovich, E. Gateff, C. Zettervall, D. Hultmark, and I. Ando, “Nimrod, a Putative Phagocytosis Receptor With Egf Repeats in Drosophila Plasmatocytes,” CURRENT BIOLOGY, vol. 17, no. 7, pp. 649–654, 2007.
  51. Medzihradszky, Z. Darula, E. Perlson, M. Fainzilber, R. Chalkley, H. Ball, D. Greenbaum, M. Bogyo, D. Tyson, R. Bradshaw, and A. Burlingame, “O-sulfonation of serine and threonine – Mass spectrometric detection and characterization of a new posttranslational modification in diverse proteins throughout the eukaryotes,” MOLECULAR & CELLULAR PROTEOMICS, vol. 3, pp. 429–440, 2004.
  52. Perlson, K. Medzihradszky, Z. Darula, D. Munno, N. Syed, A. Burlingame, and M. Fainzilber, “Differential proteomics reveals multiple components in retrogradely transported axoplasm after nerve injury,” MOLECULAR & CELLULAR PROTEOMICS, vol. 3, pp. 510–520, 2004.
  53. Kupihar, G. Kovacs, Z. Kele, Z. Darula, and L. Kovacs, “A novel and convenient method for the synthesis of free 5 ’- thiol modified oligonucleotides,” NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, vol. 22, pp. 1297–1299, 2003.
  54. Greenbaum, A. Baruch, L. Hayrapetian, Z. Darula, A. Burlingame, K. Medzihradszky, and M. Bogyo, “Chemical approaches for functionally probing the proteome,” MOLECULAR & CELLULAR PROTEOMICS, vol. 1, pp. 60–68, 2002.
  55. Halab, J. Becker, Z. Darula, D. Tourwe, B. Kieffer, F. Simonin, and W. Lubell, “Probing opioid receptor interactions with azacycloalkane amino acids. Synthesis of a potent and selective ORL1 antagonist,” JOURNAL OF MEDICINAL CHEMISTRY, vol. 45, pp. 5353–5357, 2002.
  56. Szikra, S. Benyhe, G. Orosz, Z. Darula, J. Piot, I. Fruitier, K. Monory, J. Hanoune, and A. Borsodi, “Radioligand binding properties of VV-hemorphin 7, an atypical opioid peptide,” BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 281, pp. 670–677, 2001.
  57. Darula, K. Kover, K. Monory, A. Borsodi, E. Mako, A. Ronai, D. Tourwe, A. Peter, and G. Toth, “Deltorphin II analogues with 6-hydroxy-2-aminotetralin-2-carboxylic acid in position 1,” JOURNAL OF MEDICINAL CHEMISTRY, vol. 43, no. 7, pp. 1359–1366, 2000.
  58. Darula, G. Torok, G. Wittmann, E. Mannekens, K. Iterbeke, G. Toth, D. Tourwe, and A. Peter, “A rapid, qualitative thin-layer chromatographic method for the separation of the enantiomers of unusual aromatic amino acids,” JPC – JOURNAL OF PLANAR CHROMATOGRAPHY – MODERN TLC, vol. 11, no. 5, pp. 346–349, 1998.
  59. Darula, A. Peter, and G. Toth, “Tritiated deltorphin analogues with high specific radioactivity and high affinity and selectivity for delta opioid receptors,” JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, vol. 39, no. 10, pp. 817–826, 1997.
  60. Gyires, A. Ronai, T. Toth, Z. Darula, and S. Furst, “Analysis of the role of delta opioid receptors in gastroprotection in the rat,” LIFE SCIENCES, vol. 60, no. 16, pp. 1337–1347, 1997.
  61. SPETEA, Z. DARULA, G. TOTH, and A. BORSODI, “Synthesis and binding characteristics of the highly selective radiolabelled deltorphin analogues containing 2-aminotetralin-2-carboxylic acid and position 3.,” NEUROPEPTIDES, vol. 31, pp. 483–488, 1997.
  62. Toth, Z. Darula, A. Peter, F. Fulop, D. Tourwe, H. Jaspers, P. Verheyden, Z. Bocskey, Z. Toth, and A. Borsodi, “Conformationally constrained deltorphin analogs with 2-aminotetralin-2-carboxylic acid in position 3,” JOURNAL OF MEDICINAL CHEMISTRY, vol. 40, no. 6, pp. 990–995, 1997.


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