HCEMM-SU In Vivo Imaging Advanced Core Facility

Advanced Core Facility Introduction

Advanced Core Facility Description

Head of ACF: Domokos Máthé DVM, PhD

The In Vivo Imaging Advanced Core Facility provides services to

-all HCEMM Research Groups,
-HCEMM Member University-affiliated PIs
-and external academic or industrial parties, too.

We help you to cover the full range of quantitative data generated using most in vivo imaging modalities available nowadays.

We strive to include everything: from the generation of any live animal-related measurement ideas, through advice on contrast material use or metabolic imaging measurement types.

Our service extends to the development of radiomic outputs and statistical analysis of data.

Our constantly improving and quite comprehensive array of experience, animal model choices available, and imaging systems cover small rodents (CNS, tumour and stem cell use models) and large animals (dogs, swine) as well.

Use cases of the In Vivo Imaging ACF:

-Tg organism phenotyping via visualisation of reporter gene expression (e.g. -Luc,- mCherry, mKate)

-Cell knock-in or knock-out phenotyping using reporter gene approaches and measurement of cell tracking in live animals

-Measurements of protein expression in quantitative manner using PET and SPECT

-Quantitative measurement of IC50 and Kd values for specific protein/antibody and receptor agonist/antagonist molecules using hybrid SPECT/MRI and PET/MRI imaging

-Efficacy measurements of drugs or cell-based therapies in CNS, bone/joint and tumour models using imaging readouts from fluorescence, SPECT, PET, MRI, CT or Ultrasound data

-Deciphering of CNS perfusion networks (fUS) and

-Tumour or inflammatory perfusion using ultra-high resolution ultrasound systems

-Proof-of-concept trials in large animal spontaneous disease (inflammatory bowel disease, lung fibrosis, and spontaneous tumours) by SPECT/PET/CT readouts

-Directly translational studies from the patient bed to bench and back to clinical trial: CAR-T cell therapy, radionuclide therapy and tumour radioembolisation

-Biodistribution measurements of fluorescent proteins/constructs, radiolabeling of proteins, quantitation of biodistribution with femtomol/g tissue sensitivity using PET and SPECT

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Available Sites and Instrumentation:

The IVI ACF is located in three sites, the headquarters being the Basic Medical Sciences Building at the Semmelweis University, 37-43 Tűzoltó utca, 1094 Budapest. The ACF HQ comprises two isotope laboratories and three imaging laboratory rooms. Permissions are in place to handle more than 80 isotopes for tracing, imaging and therapeutics and X-ray application. In addition, a local isotope-containing animal facility is also associated with the ACF. Two other sites are also involved, the Cardiovascular Centre (68 Városmajor u, 1122 Budapest) and the Basic Sciences Centre (4 Nagyvárad tér, 1082).

  • Equipment for Quantitative Functional Molecular Imaging
  • Small Animal Ultrahigh Resolution Optoacoustics and Doppler Ultrasound Imaging System: Iconeus ONE (Iconeus, France)
  • PET/MRI system: nanoScan PM 3T (Mediso, Hungary)
  • SPECT/CT system: nanoScan SPECT/CT 4R (Mediso, Hungary)
  • Fast and easy MRI system: nanoScan 1T (Mediso, Hungary)
  • Small Animal Ultrahigh Resolution Ultrasound System: Vevo 3100 (Fujifilm, Canada)
  • Optical Imaging Systems: FOBI (Neoscience, Korea);
  • Translational High Resolution Ultrasound System: Vivid E95 (GE, US)
  • Dedicated Clinical SPECT/PET/CT System for large animals: AnyScan TRIO (Mediso, Hungary)
  • Equipment for Structural Micro-Imaging
    • Ex vivo Micro-CT System: Skyscan 1272 (Bruker, Belgium)

Repertoire of Imaging Possibilities by Imaging Methods:

  • High Resolution MicroCT imaging Ex Vivo:
    • The platform provides resolution not possible with live imaging due to the confounding effect of even minute movements in live animals.
    • Microscopy resolution imaging on all hard and soft tissues
    • Assessment of disease progression
    • Fast reconstruction of CT images
  • SPECT/CT applications
    • Biomarker identification
    • Spatial and temporal measurement of thyroid, cardiac, hepatic and kidney functions
    • Bio-distribution and -availability of isotope-labeled theragnostic molecules already in clinical use or under development
    • Imaging of stem cell functions
  • PET/MRI multimodal applications
    • High spatial resolutions: 100 μm (MRI), 700 μm (PET) – Cellular, subcellular, and molecular identification
    • High sensitivity: femtoM/mg tissue
    • Anatomical localization, exact morphology of metabolic foreground
    • Radiolabeling-based assessment of tissue metabolic processes
    • Special focus in neurotransmitter, oncology, regeneration and immune-system studies
  • Fluorescent Imaging
    • Very high throughput easy-to-use phenotyping of reporter animals without the need of external luciferase injection and ATP access to tissue
    • Identification of autofluorescence-related processes
    • Tumor and cell tracking and time-series imaging of tumor bio-distribution or cell-based therapies
  • Ultra-high-frequency and 4D ultrasound imaging applications
    • A platform with portable ultrasound unit operating from 10 up to 70MHz
    • Applicable for mice, rats, and other larger animals
    • Focused, but not limited to, cardiovascular applications
    • fUS: High-resolution quantitation of cerebral blood flow in mice and rats for the fast, on-the-fly analysis of blood flow changes in stroke, inflammation, intestinal microbiome and cardiovascular model mice.

7. Alzheimer’s Disease: A Molecular View of β-Amyloid Induced Morbific EventsProtection in Bone Marrow Chimeric Mice

Rajmohamed Mohamed Asik, Natarajan Suganthy, Mohamed Asik Aarifa, Arvind Kumar, Krisztián Szigeti, Domokos Mathe, Balázs Gulyás, Govindaraju Archunan, Parasuraman Padmanabhan
Journal/Proc./Book: Biomedicines (9/9)
Year: 2021


6. Age-Related Inflammatory Balance Shift, Nasal Barrier Function, and Cerebro-Morphological Status in Healthy and Diseased Rodents

Zsófia Varga-Medveczky, Noémi Kovács, Melinda E. Tóth, Miklós Sántha, Ildikó Horváth, Luca Anna Bors, Katalin Fónagy, Timea Imre, Pál Szabó, Domokos Máthé, Franciska Erdő
Journal/Proc./Book: Frontiers in Neuroscience (15)
Year: 2021


5. Intraperitoneal Glucose Transport to Micrometastasis: A Multimodal In Vivo Imaging Investigation in a Mouse Lymphoma Model

 Zsombor Ritter, Katalin Zámbó, Xinkai Jia, Dávid Szöllősi, Dániel Dezső, Hussain Alizadeh, Ildikó Horváth, Nikolett Hegedűs, David Tuch, Kunal Vyas, Péter Balogh, Domokos Máthé, Erzsébet Schmidt
Year: 2021



4. Nanotheranostic agents for neurodegenerative diseases

Parasuraman Padmanabhan, Mathangi Palanivel, Ajay Kumar, Domokos Máthé, George K. Radda, Kah-Leong Lim, Balázs Gulyás
Journal/Proc./Book: Emerging Topics in Life Sciences (4/6)
Year: 2020


3. Suppression of Metastatic Melanoma Growth in Lung by Modulated
Electro-Hyperthermia Monitored by a Minimally Invasive Heat Stress
Testing Approach in Mice

Mbuotidem Jeremiah Thomas, Enikő Major, Anett Benedek, Ildikó Horváth, Domokos Máthé, Ralf Bergmann, Attila Marcell Szász, Tibor Krenács, Zoltán Benyó
Journal/Proc./Book: Cancers (12/12)
Year: 2020


2. Fluorescent, Prussian Blue-Based Biocompatible Nanoparticle System for Multimodal Imaging Contrast

László Forgách, Nikolett Hegedűs, Ildikó Horváth, Bálint Kiss, Noémi Kovács, Zoltán Varga, Géza Jakab, Tibor Kovács, Parasuraman Padmanabhan, Krisztián Szigeti, Domokos Máthé
Journal/Proc./Book: Nanomaterials (10/9)
Year: 2020


1. Thrombocytosis and Effects of IL-6 Knock-Out in a Colitis-Associated Cancer Model

Valeria Josa, Szilamer Ferenczi, Rita Szalai, Eniko Fuder, Daniel Kuti,
Krisztina Horvath, Nikolett Hegedus, Tibor Kovacs, Gergo Bagamery,
Balazs Juhasz, Zsuzsanna Winkler, Daniel S. Veres, Zsombor Zrubka,
Domokos Mathe, Zsolt Baranyai
Journal/Proc./Book: International Journal of Molecular Sciences (21/17)
Year: 2019

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